|
KMID : 0624620080410100699
|
|
BMB Reports
2008 Volume.41 No. 10 p.699 ~ p.704
|
|
|
Neuregulin-1 promotes cardiomyocyte differentiation of genetically engineered embryonic stem cell clones
|
|
Wang Zhi
Xu Guotong
Wu Yalan
Liu Shaowen
Sun Baogui
Dai Qiuyan
|
|
|
Abstract
|
|
|
|
Embryonic stem (ES) cell-derived cardiomyocytes (ESCMs) must be specifically purified in order to prevent teratoma formation, and this confusing issue has hampered their clinical application. We therefore investigated a technique to generate pure labeled ESCMs for possible use in cardiac repair. We generated transgenic ES cell lines expressing enhanced green fluorescent protein (EGFP) under the transcriptional control of the α-cardiac myosin heavy chain (α-MHC) promoter. Differentiated EGFP-positive ES cells displayed characteristics of CMs. Furthermore, neuregulin-1 (NRG-1) upregulated the expression of the cardiac-restricted transcription factors Nkx2.5 and GATA-4, as well as differentiated CM factors (α-MHC, β-MHC). Immunohistochemistry demonstrated that NRG-1 increased expression of cardiac-specific troponin T in the beating foci of the embryoid bodies. This work revealed a potential method for specifically labeling and enriching ESCMs by combining genetically-engineered ES cell clones and exogenous growth factor treatment.
|
|
|
KEYWORD
|
|
|
Cardiomyocytes, Embryonic stem cells, Enhanced green fluorescent protein, Neuregulin-1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|